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To FACS specialists

Can anyone recommend good FACS training (especially in the area of multicolor compensation)?

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( 5 comments — Leave a comment )
sciuro
May. 8th, 2012 02:28 am (UTC)
Am I correct that you are somewhere in Boston area? If yes, then BD provides on-site training in Billerica and it's awesome. Our flow core director (and a good friend of mine) just got that training (multicolor panel design etc) and I had an opportunity to glance over all her class materials - seems fantastic. I want to take it if my boss pays for it :)

How proficient is your potential trainee? Does he/she needs to obtain basic knowledge of compensation/panel design/flowjo, or we are talking about custom configuration, multi-laser optimizations and such?

(sorry, I can talk about FACS for HOURS :))
kushka
May. 8th, 2012 02:54 am (UTC)
Perfect!! I'll look it up. This is for me, and I have very basic hand on training. Years ago I took beginners training at SKI, but all the rest was intuitive learning. It was enough for one-two color jobs. Now I am starting on a project that involves 4-6 color FACS with uneven cell population.
My major problem is compensation --I asked collegue who is considered expert at this to show how properly compensate and it still looks too subjective to me. Answer -- this is better because it looks better is not enough. I need more explanations.
In addition it would be nice to get some training on flowjo. We use diva for reading samples, and flowjo -- for analysis. I use flowjo a lot, and know basics, but I am sure there are tricks that can make my life a bit easier and could save me time.
Let me know if there is a particular course you can recommend. Thank you! ((I am afraid it was wrong for you to say about your live to FACS as I will be spamming you with questions :))
sciuro
May. 8th, 2012 11:24 am (UTC)
Well, with <6 colors in mind and flowjo as a software of choice, BD might appear to be an overkill. They are very good, but they will not really give you any flowjo advice because they promote their own Diva package instead. Still, if you expect to expand to the big scope flow projects, they are the best.

Flowjo does local seminars too. They don't have an office in Boston, but their specialist/representative is often around. You may email them and ask when and where is the next seminar in Boston area (i.e. we hold one couple of times a year) and sneak in.

I personally do most of my compensation in flowjo, I don't even bother with Diva and pre-acquisition tuning.

(elevator pitch)
I am occasionally doing flow training in our own institution, and I can do flowjo in my sleep. Want to have a training session with me in exchange for the informational interview? I can explain you the basic approach and do some troubleshooting for you in 2-3 hours.
(end of elevator pitch)
:)
kushka
May. 8th, 2012 08:08 pm (UTC)
I agree about BD. They do have webseminar on compensation in Diva.
I like your offer ) Let's talk offline olga_sin yahoo
I'll email u my phone #
sciuro
May. 8th, 2012 10:03 pm (UTC)
Done :)
( 5 comments — Leave a comment )